What we do in the lab: myocyte isolation and live cell imaging!

a mouse heart perfused via aorta

ventricular myocyte shortening

Intracellular calcium dynamics

key words: cardiac physiology, calcium channel, ryanodine receptor, mitochondria, CPVT, triggered arrhythmias, heart failure, electrophysiology, gene therapy.

Publications

Arpita Deb#, Brian D. Tow#, You Qing#, Madelyn Walker, James A. Stewart Jr., Björn C. Knollmann, Yi Zheng, Ying Wang, and Bin Liu. Genetic inhibition of mitochondrial permeability transition pore exacerbates ryanodine receptor 2 dysfunction in arrhythmic disease. Cells. 2023 12:204. (#, equal contribution) (impact factor: 7.67)
Shachinthaka D. Dissanayaka Mudiyanselage, Junfei Ma, Tibor Pechan, Olga Pechanova, Bin Liu, and Ying Wang. A remodeled RNA polymerase II complex catalyzing viroid RNA-templated transcription. PLoS Pathog. 2022 Sep 19; 18(9):e1010850. (impact factor: 7.01)

3. Junfei Ma, Shachinthaka D. Dissanayaka Mudiyanselage, Woong June Park, Mo Wang, Ryuta Takeda, Bin Liu, and Ying Wang. A nuclear import pathway exploited by pathogenic noncoding RNAs. Plant Cell. 2022 34:3543-3556. (impact factor: 12.09)

4. Brian D. Tow, Arpita Deb, Shraddha Neupane, Shuchi Patel, Meagan Reed, Anna-Beth Loper, Roman A. Eliseev, Björn C. Knollmann, Sándor Györke and Bin Liu. SR-mitochondria crosstalk shapes Ca signaling to impact pathophenotype in disease models marked by dysregulated intracellular Ca release. Cardiovasc Res. 2022 Oct 21; 118(13):2819-2832. (impact factor: 13.08)

5. Ying Wang#, Bin Liu#. Circular RNA in Diseased Heart. Cells. 2020 May 17;9(5):1240. doi: 10.3390/cells9051240. (# co-corresponding author) (impact factor: 7.67)

6. Bin Liu#, Qing Lou, Heather Smith, Florencia Velez-Cortes, Wolfgang Dillmann, Björn Knollmann, Antonis Armoundas, Sandor Gyorke#. Conditional Up-Regulation of SERCA2a Exacerbates RyR2-Dependent Ventricular and Atrial Arrhythmias. Int J Mol Sci. 2020 Apr 5;21(7):E2535. doi: 10.3390/ijms21072535. (# co-corresponding author) (impact factor: 6.2)

7. Ingrid Bonilla, Andriy Belevych, Stephen Baine, Andrei Stepanov, Louisa Mezache, Tom Bodnar, Bin Liu, Pompeo Volpe, Silvia Priori, Noah Weisleder, Galina Sakuta, Cynthia Carnes, Przesmyslaw Radwanski, Rengasayee Veeraraghavan, and Sandor Gyorke. Enhancement of Cardiac Store Operated Calcium Entry (SOCE) within Novel Intercalated Disk Microdomains in Arrhythmic Disease. Sci Rep. 2019 Jul 15;9(1):10179. doi: 10.1038/s41598-019-46427-x. (impact factor: 4.99)

v11040324. (# co-corresponding author) (impact factor: 5.82)

9. Bin Liu#, Shane D#. Walton, Hsiang-Ting Ho, Andriy Belevych, Svetlana Tikunova, Ingrid Bonilla, Vikram Shettigar, Bjorn C. Knollmann, Silvia G. Priori, Pompeo Volpe, Przemysław B. Radwański, Jonathan Davis, and Sándor Györke. Gene transfer of engineered calmodulin alleviates ventricular arrhythmias in a calsequestrin-associated mouse model of catecholaminergic polymorphic ventricular tachycardia. J Am Heart Assoc. 2018 May 15; 7(10): e008155. (# equal contribution) (impact factor: 6.11)

10. Györke S, Belevych AE, Liu B, Kubasov IV, Carnes CA, Radwański PB. The role of luminal Ca regulation in Ca signaling refractoriness and cardiac arrhythmogenesis. J Gen Physiol. 2017 Sep 4;149(9):877-888. (impact factor: 4)

11. Siddiqui JK, Tikunova SB, Walton SD, Liu B, Meyer M, de Tombe PP, Neilson N, Kekenes-Huskey PM, Salhi HE, Janssen PM, Biesiadecki BJ, Davis JP. Myofilament Calcium Sensitivity: Consequences of the Effective Concentration of Troponin I. Front Physiol. 2016 Dec 21;7:632. (impact factor: 4.76)

12. Ho HT, Belevych AE, Liu B, Bonilla IM, Radwański PB, Kubasov IV, Valdivia HH, Schober K, Carnes CA, Györke S. Muscarinic Stimulation Facilitates Sarcoplasmic Reticulum Ca Release by Modulating Ryanodine Receptor 2 Phosphorylation Through Protein Kinase G and Ca/Calmodulin-Dependent Protein Kinase II. Hypertension. 2016 Nov;68(5):1171-1178. (impact factor: 9.9)

13. Radwański PB, Ho HT, Veeraraghavan R, Brunello L, Liu B, Belevych AE, Unudurthi SD, Makara MA, Priori SG, Volpe P, Armoundas AA, Dillmann WH, Knollmann BC, Mohler PJ, Hund TJ, Györke S. Neuronal Na+ Channels Are Integral Components of Pro-arrhythmic Na+/Ca2+ Signaling Nanodomain That Promotes Cardiac Arrhythmias During β-adrenergic Stimulation. JACC Basic Transl Sci. 2016 Jun;1(4):251-266 (impact factor: 9.53)

14. Davis JP, Shettigar V, Tikunova SB, Little SC, Liu B, Siddiqui JK, Janssen PM, Ziolo MT,Walton SD. Designing proteins to combat disease: Cardiac troponin C as an example. Arch Biochem Biophys. 2016 Jul 1;601:4-10. doi: 10.1016/j.abb.2016.02.007. (impact factor: 4.11)

15. Liu B, Ho HT, Brunello L, Unudurthi SD, Lou Q, Belevych AE, Qian L, Kim do H, Cho C, Janssen PM, Hund TJ, Knollmann BC, Kranias EG, Györke S. Ablation of HRC alleviates cardiac arrhythmia and improves abnormal Ca handling in CASQ2 knockout mice prone to CPVT. Cardiovasc Res. 2015 Nov 1;108(2):299-311. doi: 10.1093/cvr/cvv222. Epub 2015 Sep 25. (impact factor: 13.08)

16. Lou Q, Belevych AE, Radwański PB, Liu B, Kalyanasundaram A, Knollmann BC, Fedorov VV, Györke S. Alternating membrane potential/calcium interplay underlies repetitive focal activity in a genetic model of calcium-dependent atrial arrhythmias. J Physiol. 2015 Mar 15;593(6):1443-58. doi: 10.1113/jphysiol.2014.280784. Epub 2014 Dec 2. (impact factor: 6.23)

17. Curran J, Makara MA, Little SC, Musa H, Liu B, Wu X, Polina I, Alecusan JS, Wright P, Li J, Billman GE, Boyden PA, Gyorke S, Band H, Hund TJ, Mohler PJ. EHD3-Dependent Endosome Pathway Regulates Cardiac Membrane Excitability and Physiology. Circ Res. 2014 Jun 20;115(1):68-78.doi:10.1161/CIRCRESAHA.115.304149. (impact factor: 23.21)

18. Liu B, Ho HT, Velez-Cortes F, Lou Q, Valdivia C, Knollmann B, Valdivia H, Gyorke S. Genetic ablation of ryanodine receptor 2 phosphorylation at Ser-2808 aggravates Ca2+-dependent cardiomyopathy by exacerbating diastolic Ca2+ release. J Physiol. 2014 May 1;592(Pt 9):1957-73. doi: 10.1113/jphysiol.2013.264689. (impact factor: 6.23)

19. Liu B, Lopez JJ, Biesiadecki BJ, Davis JP. Protein kinase C phosphomimetics alter thin filament Ca2+ binding properties. PLoS One. 2014 Jan 22;9(1):e86279. (impact factor: 3.75)

20. Ho HT, Liu B, Snyder JS, Lou Q, Brundage EA, Velez-Cortes F, Wang H, Ziolo MT, Anderson ME, Sen CK, Wehrens XH, Fedorov VV, Biesiadecki BJ, Hund TJ, Györke S. Ryanodine receptor phosphorylation by oxidized CaMKII contributes to the cardiotoxic effects of cardiac glycosides. Cardiovasc Res. 2014 Jan 1;101(1):165-74. (impact factor: 13.08)

21. Nixon BR, Liu B, Scellini B, Tesi C, Piroddi N, Ogut O, John Solaro R, Ziolo MT, Janssen PM,Davis JP, Poggesi C, Biesiadecki BJ. Tropomyosin Ser-283 pseudo-phosphorylation slows myofibril relaxation. Arch Biochem Biophys. 2013 Jul 1;535(1):30-8 (impact factor: 4.11)

22. Bonilla IM, Belevych AE, Sridhar A, Nishijima Y, Ho HT, He Q, Kukielka M, Terentyev D, Terentyeva R, Liu B, Long VP, Györke S, Carnes CA, Billman GE. Endurance exercise training normalizes repolarization and calcium-handling abnormalities, preventing ventricular fibrillation in a model of sudden cardiac death. J Appl Physiol. 2012 Dec;113(11):1772-83. (impact factor: 3.88)

23. Liu B, Tikunova SB, Kline KP, Siddiqui JK, Davis JP. Disease-Related Cardiac Troponins Alter Thin Filament Ca2+ Association and Dissociation Rates. PLoS One. 2012;7(6):e38259. (impact factor: 3.75)

24. Liu B, Lee RS, Biesiadecki BJ, Tikunova SB, Davis JP. Engineered Troponin C Constructs Correct Disease-related Cardiac Myofilament Calcium Sensitivity. J Biol Chem. 2012 Jun 8;287(24):20027-36. (impact factor: 5.49)

25. Tikunova SB#, Liu B#, Swindle N, Little SC, Gomes AV, Swartz DR, Davis JP. Effect of Calcium-sensitizing Mutations on Calcium Binding and Exchange with Troponin C in Increasingly Complex Biochemical Systems. Biochemistry. 2010 Mar 9;49(9):1975-84. (# equal contribution) (impact factor: 3.32)